As many of you know, microplate readers are instruments used to detect biological, chemical, biochemical or physical events from samples in a microplate. This is where it all starts for your basic research, assay development and therapeutic research. A powerful and reliable plate reader is what you need for your scientific breakthroughs or delivering new drugs and therapeutic treatments to market. It is a crucial component and workhorse for every lab to identify the ideal drug compound to advance it from discovery to development to manufacturing.
Industries that can benefit:
Configurable Multimode Plate Readers to Future Proof Your Lab
Multimode plate readers have become very popular. Market demand has driven instrument manufacturers to progress from producing single-mode plate readers to those with multiple modes of detection. They offer flexibility and savings in cost and precious lab space, together with the convenience of employing only one instrument versus having to purchase multiple dedicated instruments.
Our multimode microplate readers offer all major detections such as Absorbance, Alpha, Luminescence, Fluorescence Intensity, Fluorescence Polarization, and Time-Resolved Fluorescence, all in one instrument. In addition to the detection technologies, our EnSight multimode plate reader also offers fast well-imaging so you can perform target-based and phenotypic screening to gain valuable insights from different perspectives. All of our plate readers are configurable so you can add technologies when you need to future proof your lab. They are ideal for applications such as cell viability/proliferation/toxicity, drug screening, pathway analysis, receptor panning, biomarkers, protein:protein interaction, gene expression, food monitoring, and epigenetics.
PerkinElmer’s portfolio of plate readers is equipped with the latest detection technologies to meet the diverse assay requirements of labs today. With over 30 years of experience in instrument development, you can expect performance and reliability from any of our microplate readers.
Software Designed for 21 CFR Part 11 Compliance
All our plate readers offer the Enhanced Security software option. It is your technological tool to help you reach compliance for 21 CFR Part 11 with features such as user management and more. Watch our short video to learn more about the Enhanced Security software option for the VICTOR? Nivo? multimode plate reader.
Absorbance is the measurement between the amount of light being absorbed by a sample and the light that passes through the sample (non-absorbed). A specific wavelength of light is usually selected from an optical filter or the whole spectrum can be captured using a spectrometer. It is one of the most well-established microplate assay formats and perfect for assays such as ELISA, protein and nucleic acid quantification, and enzyme activity.
Assay for Virtually Any Application
Alpha is a high-performance laser-based technology, validated for use with our proprietary AlphaScreen? and AlphaLISA? assay kits. It has the power to assay the most complex samples – all in one well, with no wash steps. Alpha Technology enables fast, simple, highly sensitive detection of biomolecules in cell lysates, cell supernatants, serum, and a variety of other sample types. Laser-based Alpha detection allows you to have quick readouts, while maintaining a high signal-to-background ratio.
Ideal for Reporter Gene, Cytotoxicity, and Proliferation Assays
Luminescence is the measurement of light emitted as a result of a chemical or biochemical reaction with no excitation energy. A luminescence assay is extremely useful as a detection platform because of its wide dynamic range, greater sensitivity than fluorescence technologies and lower interference than other detection options. Luminescence assay formats include glow and BRET, and flash or dual glow – perfect for applications such as reporter gene, cytotoxicity, and proliferation assays.
Popular Light Emission Detection
Fluorescence Intensity is the measurement of light emitted from a molecule (fluorophore) at one wavelength and light excited at a shorter wavelength. It is one of the most popular detection modes, and there are innumerable fluorophores available for applications such as DNA or protein quantitation, reporter-gene expression, and protein binding.
Ideal Detection for High Throughput
Similar to Fluorescence Intensity, Fluorescence Polarization is based on the emission of light by an excited fluorophore. For this detection, samples are excited by polarized light using specific filters. It is generally applied to measure binding events between small and large molecules. Fluorescence Polarization is a homogeneous assay technology that’s ideal for high-throughput screening. Fluorescein is the commonly used fluorescent label and is suitable for typical applications such as receptor-ligand binding, protein interaction, or hapten immunoassays.
Enhanced Sensitivity and Dynamic Range for Immunoassays
Time-Resolved Fluorescence (TRF) uses fluorescent molecules, called lanthanides, that can emit light over long periods of time after excitation. Time-Resolved Fluorescence can help improve the sensitivity and dynamic range of your immunoassays, even when sample is at a premium or in low concentration. TRF detection, together with lanthanide-based DELFIA, LANCE Ultra, or HTRF chemistries, offers enhanced signal-to-background ratio, high sensitivity, wide dynamic range, superior stability, and excellent flexibility.
VICTOR? Nivo? packs all the latest major detection technologies in the industry’s smallest benchtop footprint. The perfect microplate reader for everyday biochemical and cell-based assays. It measures at any wavelength, with top and bottom reading for most standard technologies. With its ease of use software and remote access from almost any device, it is ideal for multiuser environments.
EnSight? offers both fast well-imaging and multimode detection capabilities for target-based and phenotypic screening, all in a single benchtop instrument. It allows you to gain insights you couldn’t achieve with detection measurements alone. When combined with our Kaleido? workflow software, it increases productivity, makes it ideal for multiuser environments, and elevates the confidence in your scientific results.
EnVision? is our fastest benchtop multimode plate reader and it provides ultra-high throughput and maximum sensitivity across all detection technologies. With its exceptional speed and minimal downtime, it is ideal for complex assays to drive your scientific breakthroughs. In addition, our Enhanced Security software for regulated environments (GxP) will simplify your road to compliance so you can focus on the science.
|UV-Vis Absorbance||Filter or spectrometer||Filter and quad monochromators||Filter and/or quad monochromators|
|Fluorescence Intensity||Filter||Quad monochromator||Filter and/or quad monochromators|
|TRF and TR-FRET||Lamp based2||Lamp based||Lamp or laser based2|
|Alpha (Laser-Based)||Alpha Standard||Alpha HTS||Alpha Standard or Alpha HTS|
|AlphaPlex (Laser-Based)||Alpha Standard|
|Fast Image-based Cytometry||?|
|Dual PMT Detector||?|
Drug discovery is a time-consuming and costly undertaking. From understanding pathways to target identification and then to hit and eventually compound, the transition needs to happen quickly and efficiently. Our drug discovery reagents help you do just that. Whether you are studying basic science to uncover pathways and learn more about disease states or screening for potential leads, we offer a wide range of validated reagents and kits in Alpha chemiluminescent, LANCE® Ultra TR-FRET, DELFIA® TRF, and other assay technologies to suit your assay needs. Together with our multimode microplate readers, they can provide relevant and reliable results to accelerate your drug discovery.
A microplate is a critical part of your discovery process. Microplates have the capability to generate valuable, information-rich data that bring great insights into discovery screening.
Microplate planarity and inaccurate data collection are common issues caused by microplates that are not carefully designed for the unique challenges of sample screening which can result in missed data points, sample re-screening, missed project timelines, and ultimately higher costs.